Using the Cre-lox system to randomize target gene expression states and generate diverse phenotypes.

ABSTRACT: Modifying the expression of multiple genes enables both deeper understanding of their function and the engineering of complex multigenic cellular phenotypes. However, deletion or overexpression of multiple genes is typically laborious and involves multiple sequential genetic modifications. Here we describe a strategy to randomize the expression state of multiple genes in Saccharomyces cerevisiae using Cre-loxP recombination. By inserting promoters flanked by inverted loxP sites in front of a gene of interest we can randomly alter its expression by turning it OFF or ON, or between four distinct expression states. We show at least 6 genes can be randomized independently and argue that using orthogonal loxP sites should increase this number to at least 15. Finally, we show how combining this

SUBMITTER: Niesner B 

PROVIDER: S-EPMC4264681 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature