Discovery of rare mutations in extensively pooled DNA samples using multiple target enrichment.

ABSTRACT: Chemical mutagenesis is routinely used to create large numbers of rare mutations in plant and animal populations, which can be subsequently subjected to selection for beneficial traits and phenotypes that enable the characterization of gene functions. Several next-generation sequencing (NGS)-based target enrichment methods have been developed for the detection of mutations in target DNA regions. However, most of these methods aim to sequence a large number of target regions from a small number of individuals. Here, we demonstrate an effective and affordable strategy for the discovery of rare mutations in a large sodium azide-induced mutant rice population (F2 ). The integration of multiplex, semi-nested PCR combined with NGS library construction allowed for the amplification of multiple ta

SUBMITTER: Chi X 

PROVIDER: S-EPMC4265296 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature