An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis.

ABSTRACT:

Background

Scientific interest in Enterococcus faecalis has increased greatly over recent decades. Some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. The limited availability of vectors able to express cloned genes efficiently in E. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. The agmatine deiminase (AGDI) pathway of E. faecalis, involved in the conversion of agmatine into putrescine, is driven by a response inducer gene aguR.

Results

This study describes that the exposure to the induction factor (agmatine) results in the transcription of genes under the control of the aguB promoter, including the ag

SUBMITTER: Linares DM 

PROVIDER: S-EPMC4265343 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature