MicroRNA-1269 promotes proliferation in human hepatocellular carcinoma via downregulation of FOXO1.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the most common malignancies and a major cause of cancer-related mortality in the world. MicroRNAs (miRNAs) are small, noncoding RNAs that play essential roles in various stages during cancer progression. The aim of the current study was to elucidate the role of miR-1269 in the pathogenesis of HCC.

Methods

The expression of miR-1269 in HCC cells and tissues were determined by Real-time PCR analysis. Cell viability, colony formation and anchorage-independent growth ability assays were performed to examine cell proliferative capacity and tumorigenicity. Flow cytometry analysis was conducted to determine cell cycle progression. The expression of p21, CyclinD1, phosphorylated Rb, Rb and FOXO1 were examined by Western blotting a

SUBMITTER: Yang XW 

PROVIDER: S-EPMC4265494 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature