Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma.

ABSTRACT:

Aim

To investigate the function of Pea3 in colorectal carcinoma (CRC) invasion and metastatic potential.

Methods

The expression of Pea3 during clinical progression of human CRC was investigated using Oncomine Research Edition. To assay Pea3 expression in established CRC cell lines, we performed western blotting of cell lysates. We employed shRNA-mediated knockdown of Pea3 in HCT116 (HCT) and LS174T CRC cells which was confirmed by real-time quantitative PCR (qPCR) and western blotting. Transwell invasion assays, MTS proliferation assays, anoikis assays, and fluorometric matrix metalloprotease (MMP) assays were performed to determine the effects of Pea3 knockdown on invasion, proliferation, anoikis and MMP activity in CRC cells in vitro. Alterations in epithelial-mesenchymal

SUBMITTER: Mesci A 

PROVIDER: S-EPMC4265596 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature