Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry.

ABSTRACT: Protein-protein interactions are crucial for cellular homeostasis and play important roles in the dynamic execution of biological processes. While antibodies represent a well-established tool to study protein interactions of extracellular domains and secreted proteins, as well as in fixed and permeabilized cells, they usually cannot be functionally expressed in the cytoplasm of living cells. Non-immunoglobulin protein-binding scaffolds have been identified that also function intracellularly and are now being engineered for synthetic biology applications. Here we used the Designed Ankyrin Repeat Protein (DARPin) scaffold to generate binders to fluorescent proteins and used them to modify biological systems directly at the protein level. DARPins binding to GFP or mCherry were selected by rib

SUBMITTER: Brauchle M 

PROVIDER: S-EPMC4265764 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature