Localization of SK2 channels relative to excitatory synaptic sites in the mouse developing Purkinje cells.

ABSTRACT: Small-conductance, Ca(2+)-activated K(+) (SK) channels regulate neuronal excitability in a variety of ways. To understand their roles in different neuronal subtypes it is important to determine their precise subcellular distribution. Here, we used biochemical, light microscopy immunohistochemical and immunoelectron microscopy techniques, combined with quantitative approaches, to reveal the expression and subcellular localization patterns of SK2 in the developing cerebellum. Using western blots, the SK2 protein showed a progressive increase during postnatal development. At the light microscopic level, SK2 immunoreactivity was very prominent in the developing Purkinje cells (PC), particularly in the molecular layer (ML). Electron microscopy revealed that throughout development SK2 was mostly

SUBMITTER: Ballesteros-Merino C 

PROVIDER: S-EPMC4266016 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature