Prevalence and molecular identification of cryptosporidium spp. In pre-weaned dairy calves in mashhad area, khorasan razavi province, iran.

ABSTRACT:

Background

Cryptosporidium parvum is a zoonotic pathogen transmissible from a variety of animals to humans and is a considerable public health concern. Dairy cattle have been identified in numerous reports as a major source of environmental contamination with this pathogen. The aim of study was to detect and isolate the Cryptosporidium spp. from fecal samples of naturally infected pre-wean calves in the Mashhad area.

Methods

Overall, 300 fecal specimens from 1 to 30 days pre-weaned calves were collected from 10 farms in the Mashhad area the capital center of the Khorasan Razavi Province, Iran and microscopically examined for Cryptosporidium spp. All infected samples were also analyzed using nested -PCR. A polymerase chain reaction (PCR)-restriction fragment length polymorphi

SUBMITTER: Asadpour M 

PROVIDER: S-EPMC4266125 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature