Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling.

ABSTRACT:

Objective

To test whether bone morphogenetic protein 4 (BMP4) directly regulates differentiation of adult mouse ovary-derived oogonial stem cells (OSCs) in vitro.

Design

Animal study.

Setting

Research laboratory.

Animal(s)

Adult C57BL/6 female mice.

Intervention(s)

After purification from adult ovaries by fluorescence-activated cell sorting, OSCs were cultured without or with BMP4 in the absence or presence of the BMP4 antagonist, Noggin.

Main outcome measure(s)

Rates of in vitro-derived (IVD) oocyte formation and changes in gene expression were assessed.

Result(s)

Cultured OSCs expressed BMP receptor (BMPR) 1A (BMPR1A), BMPR1B, and BMPR2, suggesting that BMP signaling can directly affect OSC function. In agreement with this, BMP4 significa

SUBMITTER: Park ES 

PROVIDER: S-EPMC4266321 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature