IM-TORNADO: a tool for comparison of 16S reads from paired-end libraries.

ABSTRACT:

Motivation

16S rDNA hypervariable tag sequencing has become the de facto method for accessing microbial diversity. Illumina paired-end sequencing, which produces two separate reads for each DNA fragment, has become the platform of choice for this application. However, when the two reads do not overlap, existing computational pipelines analyze data from read separately and underutilize the information contained in the paired-end reads.

Results

We created a workflow known as Illinois Mayo Taxon Organization from RNA Dataset Operations (IM-TORNADO) for processing non-overlapping reads while retaining maximal information content. Using synthetic mock datasets, we show that the use of both reads produced answers with greater correlation to those from full length 16S rDNA when loo

SUBMITTER: Jeraldo P 

PROVIDER: S-EPMC4266640 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature