Light-promoted rhodopsin expression and starvation survival in the marine dinoflagellate Oxyrrhis marina.

ABSTRACT: The discovery of microbial rhodopsins in marine proteobacteria changed the dogma that photosynthesis is the only pathway to use the solar energy for biological utilization in the marine environment. Although homologs of these rhodopsins have been identified in dinoflagellates, the diversity of the encoding genes and their physiological roles remain unexplored. As an initial step toward addressing the gap, we conducted high-throughput transcriptome sequencing on Oxyrrhis marina to retrieve rhodopsin transcripts, rapid amplification of cDNA ends to isolate full-length cDNAs of dominant representatives, and quantitative reverse-transcription PCR to investigate their expression under varying conditions. Our phylogenetic analyses showed that O. marina contained both the proton-pumping type (PR)

SUBMITTER: Guo Z 

PROVIDER: S-EPMC4266641 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature