Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy.

ABSTRACT: Single molecule-based superresolution imaging has become an essential tool in modern cell biology. Because of the limited depth of field of optical imaging systems, one of the major challenges in superresolution imaging resides in capturing the 3D nanoscale morphology of the whole cell. Despite many previous attempts to extend the application of photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) techniques into three dimensions, effective localization depths do not typically exceed 1.2 µm. Thus, 3D imaging of whole cells (or even large organelles) still demands sequential acquisition at different axial positions and, therefore, suffers from the combined effects of out-of-focus molecule activation (increased background) and bleaching (los

SUBMITTER: Hajj B 

PROVIDER: S-EPMC4267334 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature