Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A₁ and A_2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats.

ABSTRACT: Background: Caffeine is the most widely consumed psychoactive substance in the world, even during pregnancy. Its stimulatory effects are mainly due to antagonism of adenosine actions by blocking adenosine A₁ and A_2A receptors. Previous studies have shown that caffeine can cross the placenta and therefore modulate these receptors not only in the fetal brain but also in the heart. Methods: In the present work, the effect of caffeine chronically consumed during pregnancy on A₁ and A_2A receptors in Wistar rat heart, from both mothers and their fetuses, were studied using radioligand binding, Western-blotting, and adenylyl cyclase activity assays, as well as reverse transcription polymerase chain reaction. Results:

SUBMITTER: Iglesias I 

PROVIDER: S-EPMC4267408 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature