Islet amyloid formation is an important determinant for inducing islet inflammation in high-fat-fed human IAPP transgenic mice.

ABSTRACT:

Aims/hypothesis

Amyloid deposition and inflammation are characteristic of islet pathology in type 2 diabetes. The aim of this study was to determine whether islet amyloid formation is required for the development of islet inflammation in vivo.

Methods

Human islet amyloid polypeptide transgenic mice and non-transgenic littermates (the latter incapable of forming islet amyloid) were fed a low-fat (10%) or high-fat (60%) diet for 12 months; high-fat feeding induces islet amyloid formation in transgenic mice. At the conclusion of the study, glycaemia, beta cell function, islet amyloid deposition, markers of islet inflammation and islet macrophage infiltration were measured.

Results

Fasting plasma glucose levels did not differ by diet or genotype. Insulin release in respon

SUBMITTER: Meier DT 

PROVIDER: S-EPMC4267509 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature