Automated Cell Detection and Morphometry on Growth Plate Images of Mouse Bone.

ABSTRACT: Microscopy imaging of mouse growth plates is extensively used in biology to understand the effect of specific molecules on various stages of normal bone development and on bone disease. Until now, such image analysis has been conducted by manual detection. In fact, when existing automated detection techniques were applied, morphological variations across the growth plate and heterogeneity of image background color, including the faint presence of cells (chondrocytes) located deeper in tissue away from the image's plane of focus, and lack of cell-specific features, interfered with identification of cell. We propose the first method of automated detection and morphometry applicable to images of cells in the growth plate of long bone. Through ad hoc sequential application of the Retinex metho

SUBMITTER: Ascenzi M 

PROVIDER: S-EPMC4267696 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature