Quantitative trait locus mapping of melanization in the plant pathogenic fungus Zymoseptoria tritici.

ABSTRACT: Melanin plays an important role in virulence and antimicrobial resistance in several fungal pathogens. The wheat pathogen Zymoseptoria tritici is important worldwide, but little is known about the genetic architecture of pathogenicity, including the production of melanin. Because melanin production can exhibit complex inheritance, we used quantitative trait locus (QTL) mapping in two crosses to identify the underlying genes. Restriction site-associated DNA sequencing was used to genotype 263 (cross 1) and 261 (cross 2) progeny at ~8500 single-nucleotide polymorphisms and construct two dense linkage maps. We measured gray values, representing degrees of melanization, for single-spore colonies growing on Petri dishes by using a novel image-processing approach that enabled high-throughput phe

SUBMITTER: Lendenmann MH 

PROVIDER: S-EPMC4267946 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature