TNFα signalling primes chromatin for NF-κB binding and induces rapid and widespread nucleosome repositioning.

ABSTRACT:

Background

The rearrangement of nucleosomes along the DNA fiber profoundly affects gene expression, but little is known about how signalling reshapes the chromatin landscape, in three-dimensional space and over time, to allow establishment of new transcriptional programs.

Results

Using micrococcal nuclease treatment and high-throughput sequencing, we map genome-wide changes in nucleosome positioning in primary human endothelial cells stimulated with tumour necrosis factor alpha (TNFα) - a proinflammatory cytokine that signals through nuclear factor kappa-B (NF-κB). Within 10 min, nucleosomes reposition at regions both proximal and distal to NF-κB binding sites, before the transcription factor quantitatively binds thereon. Similarly, in long TNFα-responsive genes, repositioni

SUBMITTER: Diermeier S 

PROVIDER: S-EPMC4268828 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature