Metagenomic evidence for metabolism of trace atmospheric gases by high-elevation desert Actinobacteria.

ABSTRACT: Previous surveys of very dry Atacama Desert mineral soils have consistently revealed sparse communities of non-photosynthetic microbes. The functional nature of these microorganisms remains debatable given the harshness of the environment and low levels of biomass and diversity. The aim of this study was to gain an understanding of the phylogenetic community structure and metabolic potential of a low-diversity mineral soil metagenome that was collected from a high-elevation Atacama Desert volcano debris field. We pooled DNA extractions from over 15 g of volcanic material, and using whole genome shotgun sequencing, observed only 75-78 total 16S rRNA gene OTUs3%. The phylogenetic structure of this community is significantly under dispersed, with actinobacterial lineages making up 97.9-98.6%

SUBMITTER: Lynch RC 

PROVIDER: S-EPMC4269115 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature