Hsa-miR-520d converts fibroblasts into CD105+ populations.

ABSTRACT:

Background

We have previously shown that hsa-miR-520d-5p can convert cancer cells into induced pluripotent stem cells (iPSCs) or mesenchymal stem cells (MSCs) via a dedifferentiation by a demethylation mechanism.

Methods

We tested the effect of miR-520d-5p on human fibroblasts to determine whether it could be safely used in normal cells for future clinical therapeutic applications. After we transfected the microRNA into fibroblasts, we analyzed the phenotypic changes, gene expression levels, and stemness induction in vitro, and we evaluated tumor formation in an in vivo xenograft model.

Results

The transfected fibroblasts turned into CD105+ cell populations, survived approximately 24 weeks, and exhibited increases in both the collagen-producing ability and in differen

SUBMITTER: Ishihara Y 

PROVIDER: S-EPMC4269822 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature