Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs.

ABSTRACT:

Background

Preliminary studies showed the prevalence of a virus similar to human hepatitis B virus (HBV-like) in swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil. In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential.

Results

Bile and liver samples from swine were positive for partial genome amplification (ORF S and ORF C), direct sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the pre

SUBMITTER: Vieira YR 

PROVIDER: S-EPMC4269919 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature