Follicle microenvironment-associated alterations in gene expression in the mouse oocyte and its polar body.

ABSTRACT:

Objective

To determine whether the follicle environment modulates oocyte-specific gene transcript levels in cultured oocytes and polar bodies (PBs).

Design

Animal study.

Setting

Large academic research center.

Animal(s)

CD1 mice.

Intervention(s)

In vitro growth of secondary mouse follicles in 0.25% or 1.5% alginate (ALG) in a three-dimensional culture system.

Main outcome measure(s)

Relative transcript levels of Gdf9, Bmp15, Nlrp5, Tcl1, and Zp3 were measured by real-time quantitative reverse transcriptase-polymerase chain reaction in oocytes during in vitro follicle development and oocyte maturation and in their first PBs after removal from metaphase II (MII) eggs.

Result(s)

All transcripts decreased earlier in oocytes cultured in 1.5% ALG

SUBMITTER: Jiao ZX 

PROVIDER: S-EPMC4270088 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature