Comparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity.

ABSTRACT:

Objective

Perform gene expression profiling of adult mouse ovary-derived oogonial stem cells (OSCs).

Design

Experimental animal study.

Setting

Research laboratory.

Animal(s)

Adult C57BL/6 female mice.

Intervention(s)

None.

Main outcome measure(s)

Gene expression profiles were compared between freshly isolated and cultured OSCs, as well as between OSCs and embryonic stem cells (ESCs), fetal primordial germ cells (PGCs), and spermatogonial stem cells (SSCs); OSC yield from ovaries versus meiotic gene activation during the estrous cycle was determined.

Result(s)

Freshly isolated OSCs, PGCs, and SSCs exhibited distinct gene expression profiles. Cultured OSCs maintained their germline gene expression pattern but gained expression of pluripotency

SUBMITTER: Imudia AN 

PROVIDER: S-EPMC4270279 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature