Universal single-probe RT-PCR assay for diagnosis of dengue virus infections.

ABSTRACT:

Background

Dengue is a mosquito-borne viral disease that has become more prevalent in the last few decades. Most patients are viremic when they present with symptoms, and early diagnosis of dengue is important in preventing severe clinical complications associated with this disease and also represents a key factor in differential diagnosis. Here, we designed and validated a hydrolysis-probe-based one-step real-time RT-PCR assay that targets the genomes of dengue virus serotypes 1-4.

Methodology/principal findings

The primers and probe used in our RT-PCR assay were designed to target the 3' untranslated region of all complete genome sequences of dengue virus available in GenBank (n = 3,305). Performance of the assay was evaluated using in vitro transcribed RNA, laboratory-ada

SUBMITTER: Alm E 

PROVIDER: S-EPMC4270494 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature