Staphylococcal enterotoxin C in synovial fluid of patients with rheumatoid arthritis.

ABSTRACT:

Background

In the previous studies using the commercial ELISA kit, the existence of staphylococcal superantigens has been reported in synovial fluid of patients with rheumatoid arthritis (RA).

Objectives

This study aimed to design molecular methods to detect staphylococcal enterotoxin C in synovial fluid of patients with rheumatoid arthritis.

Materials and methods

In this experimental study, Staphylococcus aureus strain producing enterotoxin C was used as the reference strain. The polymerase chain reaction (PCR) was set up by design a specific pair of primers. Besides bacterial culture, 50 synovial fluid samples of patients with rheumatoid arthritis were subjected to DNA extraction, and then PCR amplification was carried out according to the protocol. All samples were

SUBMITTER: Ataee RA 

PROVIDER: S-EPMC4270673 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature