Novel genotyping tools for investigating transmission dynamics of Plasmodium falciparum.

ABSTRACT:

Background

Differentiation between gametocyte-producing Plasmodium falciparum clones depends on both high levels of stage-specific transcripts and high genetic diversity of the selected genotyping marker obtained by a high-resolution typing method. By analyzing consecutive samples of one host, the contribution of each infecting clone to transmission and the dynamics of gametocyte production in multiclone infections can be studied.

Methods

We have evaluated capillary electrophoresis based differentiation of 6 length-polymorphic gametocyte genes. RNA and DNA of 25 µL whole blood from 46 individuals from Burkina Faso were simultaneously genotyped.

Results

Highest discrimination power was achieved by pfs230 with 18 alleles, followed by pfg377 with 15 alleles. When assays

SUBMITTER: Wampfler R 

PROVIDER: S-EPMC4271069 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature