Effect of miR-142-3p on the M2 macrophage and therapeutic efficacy against murine glioblastoma.

ABSTRACT:

Background

The immune therapeutic potential of microRNAs (miRNAs) in the context of tumor-mediated immune suppression has not been previously described for monocyte-derived glioma-associated macrophages, which are the largest infiltrating immune cell population in glioblastomas and facilitate gliomagenesis.

Methods

An miRNA microarray was used to compare expression profiles between human glioblastoma-infiltrating macrophages and matched peripheral monocytes. The effects of miR-142-3p on phenotype and function of proinflammatory M1 and immunosuppressive M2 macrophages were determined. The therapeutic effect of miR-142-3p was ascertained in immune-competent C57BL/6J mice harboring intracerebral GL261 gliomas and in genetically engineered Ntv-a mice bearing high-grade gliomas.

SUBMITTER: Xu S 

PROVIDER: S-EPMC4271080 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature