Molecular signatures that correlate with induction of lens regeneration in newts: lessons from proteomic analysis.

ABSTRACT:

Background

Amphibians have the remarkable ability to regenerate missing body parts. After complete removal of the eye lens, the dorsal but not the ventral iris will transdifferentiate to regenerate an exact replica of the lost lens. We used reverse-phase nano-liquid chromatography followed by mass spectrometry to detect protein concentrations in dorsal and ventral iris 0, 4, and 8 days post-lentectomy. We performed gene expression comparisons between regeneration and intact timepoints as well as between dorsal and ventral iris.

Results

Our analysis revealed gene expression patterns associated with the ability of the dorsal iris for transdifferentiation and lens regeneration. Proteins regulating gene expression and various metabolic processes were enriched in regeneration tim

SUBMITTER: Sousounis K 

PROVIDER: S-EPMC4271507 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature