Spatial and temporal features of the growth of a bacterial species colonizing the zebrafish gut.

ABSTRACT:

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The vertebrate intestine is home to microbial ecosystems that play key roles in host development and health. Little is known about the spatial and temporal dynamics of these microbial communities, limiting our understanding of fundamental properties, such as their mechanisms of growth, propagation, and persistence. To address this, we inoculated initially germ-free zebrafish larvae with fluorescently labeled strains of an Aeromonas species, representing an abundant genus in the zebrafish gut. Using light sheet fluorescence microscopy to obtain three-dimensional images spanning the gut, we quantified the entire bacterial load, as founding populations grew from tens to tens of thousands of cells over several hours. The data yield the first ever measurements of the growth k

SUBMITTER: Jemielita M 

PROVIDER: S-EPMC4271548 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature