Molecular and metabolic adaptations of Lactococcus lactis at near-zero growth rates.

ABSTRACT: This paper describes the molecular and metabolic adaptations of Lactococcus lactis during the transition from a growing to a near-zero growth state by using carbon-limited retentostat cultivation. Transcriptomic analyses revealed that metabolic patterns shifted between lactic- and mixed-acid fermentations during retentostat cultivation, which appeared to be controlled at the level of transcription of the corresponding pyruvate dissipation-encoding genes. During retentostat cultivation, cells continued to consume several amino acids but also produced specific amino acids, which may derive from the conversion of glycolytic intermediates. We identify a novel motif containing CTGTCAG in the upstream regions of several genes related to amino acid conversion, which we propose to be the target si

SUBMITTER: Ercan O 

PROVIDER: S-EPMC4272703 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature