Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis.

ABSTRACT:

Aim

To characterize how insulin-like growth factor 2 (IGF2) mRNA binding protein p62/IMP2-2 promotes steatohepatitis in the absence of dietary cholesterol.

Methods

Non-alcoholic steatohepatitis (NASH) was induced in wild-type mice and in mice overexpressing p62 specifically in the liver by feeding the mice a methionine and choline deficient (MCD) diet for either two or four weeks. As a control, animals were fed a methionine and choline supplemented diet. Serum triglycerides, cholesterol, glucose, aspartate aminotransferase and alanine transaminase were determined by standard analytical techniques. Hepatic gene expression was determined by real-time reverse transcription-polymerase chain reaction. Generation of reactive oxygen species in liver tissue was quantified as thiobar

SUBMITTER: Simon Y 

PROVIDER: S-EPMC4273134 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature