3-D Scaffold Platform for Optimized Non-viral Transfection of Multipotent Stem Cells.

ABSTRACT: Optimization of non-viral gene delivery from biomaterials is of critical importance, as several material parameters are known to influence non-viral transfection efficiency. A series of previous studies have achieved screening of gene delivery vectors on two dimensional (2D) substrates, which have direct relevance to cell culture applications. There is an additional need to create screening systems that are 3-dimensional (3D), and can thus be applied to emerging tissue engineering applications. Here, we report an enhanced throughput, 3D scaffold platform to screen for the influence of mineral coating properties on stem cell transfection. Mineral coatings with a range of physicochemical properties were formed on the scaffolds within a 96-well plate format, while maintaining an interconnecte

SUBMITTER: Yu X 

PROVIDER: S-EPMC4273581 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature