IPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.

ABSTRACT:

Background

Nuclear reprogramming inculcates pluripotent capacity by which de novo tissue differentiation is enabled. Yet, introduction of ectopic reprogramming factors may desynchronize natural developmental schedules. This study aims to evaluate the effect of imposed transgene load on the cardiogenic competency of induced pluripotent stem (iPS) cells.

Methods and results

Targeted inclusion and exclusion of reprogramming transgenes (c-MYC, KLF4, OCT4, and SOX2) was achieved using a drug-inducible and removable cassette according to the piggyBac transposon/transposase system. Pulsed transgene overexpression, before iPS cell differentiation, hindered cardiogenic outcomes. Delayed in counterparts with maintained integrated transgenes, transgene removal enabled proficient differ

SUBMITTER: Martinez-Fernandez A 

PROVIDER: S-EPMC4273857 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature