Regulated expression of PTPRJ by COX-2/PGE2 axis in endothelial cells.

ABSTRACT:

Background

This study was designed to examine a novel role of COX-2/PGE2 signaling as a regulator of PTPRJ expression in endothelial cells.

Methods

A bioinformatics analysis of a whole genome array was carried out to search for regulators of PTPRJ expression in endothelial cells. PTPRJ expression was also measured in endothelial cells derived from a balloon injury-induced neointimal hyperplasia model in male New Zealand Rabbits. Changes in PTPRJ expression in HUVEC cells was examined by RT-PCR and western blotting after transfection of COX-2 plasmids or treatment with varying concentrations of a COX-2 inhibitor.

Results

A significant correlation was identified between COX-2 and PTPRJ in GSE39264 (Pearson correlation coefficient  =  -0.87; n = 22; P < 0.01, two-tailed)

SUBMITTER: Xu X 

PROVIDER: S-EPMC4274085 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature