Genomic instability causes HGF gene activation in colon cancer cells, promoting their resistance to necroptosis.

ABSTRACT:

Background & aims

Genomic instability promotes colon carcinogenesis by inducing genetic mutations, but not all genes affected by this process have been identified. We investigated whether genomic instability in human colorectal cancer (CRC) cells produces mutations in the hepatocyte growth factor (HGF) gene.

Methods

We genotyped human colon tumor tissues and adjacent nontumor tissues collected from 78 patients University of Pittsburgh Health Sciences and Veterans Hospital, along with 40 human CRC and adjacent nontumor tissues in a commercial microarray. We used cellular, biochemical, and molecular biological techniques to investigate the factors that alter HGF signaling in colon cancer cells and its effects on cell proliferation and survival.

Results

All tested human

SUBMITTER: Seneviratne D 

PROVIDER: S-EPMC4274190 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature