Histologic identification of prominent intrapulmonary anastomotic vessels in severe congenital diaphragmatic hernia.

ABSTRACT:

Objective

To determine whether prominent intrapulmonary anastomotic vessels (IPAVs) or bronchopulmonary "shunt" vessels can be identified in lungs from infants with fatal congenital diaphragmatic hernia (CDH).

Study design

We performed histology with immunostaining for CD31 (endothelium) and D2-40 (lymphatics), along with high-precision 3-dimensional (3D) reconstruction on lung tissue from 9 patients who died with CDH.

Results

Each patient with CDH required mechanical ventilation, cardiotonic support, and pulmonary hypertension (PH)-targeted drug therapy. All patients were diagnosed with severe PH by echocardiography, and 5 received extracorporeal membrane oxygenation therapy. Death occurred at a median age of 24 days (range, 10-150 days) from refractory hypoxemia wit

SUBMITTER: Acker SN 

PROVIDER: S-EPMC4274215 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature