Dependence of cardiac transverse tubules on the BAR domain protein amphiphysin II (BIN-1).

ABSTRACT:

Rationale

Transverse tubules (t-tubules) regulate cardiac excitation-contraction coupling and exhibit interchamber and interspecies differences in expression. In cardiac disease, t-tubule loss occurs and affects the systolic calcium transient. However, the mechanisms controlling t-tubule maintenance and whether these factors differ between species, cardiac chambers, and in a disease setting remain unclear.

Objective

To determine the role of the Bin/Amphiphysin/Rvs domain protein amphiphysin II (AmpII) in regulating t-tubule maintenance and the systolic calcium transient.

Methods and results

T-tubule density was assessed by di-4-ANEPPS, FM4-64 or WGA staining using confocal microscopy. In rat, ferret, and sheep hearts t-tubule density and AmpII protein levels were lowe

SUBMITTER: Caldwell JL 

PROVIDER: S-EPMC4274343 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature