Elevated temperature alters proteomic responses of individual organisms within a biofilm community.

ABSTRACT: Microbial communities that underpin global biogeochemical cycles will likely be influenced by elevated temperature associated with environmental change. Here, we test an approach to measure how elevated temperature impacts the physiology of individual microbial groups in a community context, using a model microbial-based ecosystem. The study is the first application of tandem mass tag (TMT)-based proteomics to a microbial community. We accurately, precisely and reproducibly quantified thousands of proteins in biofilms growing at 40, 43 and 46 °C. Elevated temperature led to upregulation of proteins involved in amino-acid metabolism at the level of individual organisms and the entire community. Proteins from related organisms differed in their relative abundance and functional responses to

SUBMITTER: Mosier AC 

PROVIDER: S-EPMC4274423 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature