A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis.

ABSTRACT: Methanothermobacter marburgensis is a strictly anaerobic, thermophilic methanogenic archaeon that uses methanogenesis to convert H2 and CO2 to energy. M. marburgensis is one of the best-studied methanogens, and all genes required for methanogenic metabolism have been identified. Nonetheless, the present study describes a gene (Gene ID 9704440) coding for a putative

Nad(p)h

quinone oxidoreductase that has not yet been identified as part of the metabolic machinery. The gene product, MmNQO, was successfully expressed, purified and characterized biochemically, as well as structurally. MmNQO was identified as a flavin-dependent NADH:quinone oxidoreductase with the capacity to oxidize NADH in the presence of a wide range of electron acceptors, whereas NADPH was oxidized with only three ac

SUBMITTER: Ullmann E 

PROVIDER: S-EPMC4274662 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature