Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China.

ABSTRACT:

Background

Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings

DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corr

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4274727 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature