Antibacterial properties of Acinetobacter baumannii phage Abp1 endolysin (PlyAB1).

ABSTRACT:

Background

Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs. Endolysins from phages are attracting increasing interest as potential antimicrobial agents, especially for drug-resistant bacteria. We previously isolated and characterized Abp1, a virulent phage targeting the multidrug-resistant A. baumannii strain, AB1.

Methods

To evaluate the antimicrobial potential of endolysin from the Abp1 phage, the endolysin gene plyAB1 was cloned and over-expressed in Escherichia coli, and the lytic activity of the recombinant protein (PlyAB1) was tested by turbidity assessment and bacteria counting assays.

Results

PlyAB1 exhibits a marked lytic activity agai

SUBMITTER: Huang G 

PROVIDER: S-EPMC4274762 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature