Rat silicone hydrogel contact lens model: effects of high- versus low-Dk lens wear.

ABSTRACT:

Objectives

This study used a rat contact lens (CL) model to test if high- versus low-Dk lens wear caused changes in (1) conjunctival Langerhans cell (LC) number or location; (2) Bcl-2 expression; and (3) infection risk.

Methods

Female, Lewis rats wore a high- or low-Dk CL continuously for 2 weeks. Afterward, corneas were harvested and processed for ADPase activity to identify LCs, for immunostaining and for real time-polymerase chain reaction. Contact lens-wearing rats also were challenged with Pseudomonas aeruginosa by placing a bacterial-soaked CL on the eye followed by topical delivery of bacteria. After 48 hrs, slit lamp examination and real time-polymerase chain reaction were used to evaluate the corneal response.

Results

Conjunctival LC were significantly increa

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4274951 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature