Bone marrow regeneration promoted by biophysically sorted osteoprogenitors from mesenchymal stromal cells.

ABSTRACT: Human tissue repair deficiencies can be supplemented through strategies to isolate, expand in vitro, and reimplant regenerative cells that supplant damaged cells or stimulate endogenous repair mechanisms. Bone marrow-derived mesenchymal stromal cells (MSCs), a subset of which is described as mesenchymal stem cells, are leading candidates for cell-mediated bone repair and wound healing, with hundreds of ongoing clinical trials worldwide. An outstanding key challenge for successful clinical translation of MSCs is the capacity to produce large quantities of cells in vitro with uniform and relevant therapeutic properties. By leveraging biophysical traits of MSC subpopulations and label-free microfluidic cell sorting, we hypothesized and experimentally verified that MSCs of large diameter withi

SUBMITTER: Poon Z 

PROVIDER: S-EPMC4275011 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature