A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matrices.

ABSTRACT: Francisella tularensis, a Gram-negative bacterium and causative agent of tularemia, is categorized as a Class A select agent by the Centers for Disease Control and Prevention due to its ease of dissemination and ability to cause disease. Oropharyngeal and gastrointestinal tularemia may occur due to ingestion of contaminated food and water. Despite the concern to public health, little research is focused on F. tularensis detection in food and environmental matrices. Current diagnostics rely on host responses and amplification of F. tularensis genetic elements via Polymerase Chain Reaction; however, both tools are limited by development of an antibody response and limit of detection, respectively. During our investigation to develop an improved culture medium to aid F. tularensis diagnostics

SUBMITTER: Lamont EA 

PROVIDER: S-EPMC4275185 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature