Genome-wide ChIP-seq analysis of TCF4 binding regions in colorectal cancer cells.

ABSTRACT:

Objective

TCF4 (transcriptional factor 4) forms a complex with its transcriptional coactivator β-catenin and the coactivator carries the final signal output from the canonical Wnt signaling pathway, which is essential for the growth of normal epithelium and also plays important roles in carcinogenesis of colon epithelium. We aimed to gain a better understanding of the genes bound by TCF4 in colorectal cancer cells.

Methods

SW620 human colorectal cancer cells were cultured. The TCF4 antibody of this study was confirmed in SW620 cells by Western Blot. A ChIP-seq based genome-wide analysis of TCF4 chromatin occupancy in colorectal cancer cells was conducted and 1506 high confidence TCF4 binding sites wereidentified.

Results

Sequence analysis revealed that the binding sit

SUBMITTER: Chen C 

PROVIDER: S-EPMC4276198 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature