Viral metagenomics reveal blooms of anelloviruses in the respiratory tract of lung transplant recipients.

ABSTRACT: Few studies have examined the lung virome in health and disease. Outcomes of lung transplantation are known to be influenced by several recognized respiratory viruses, but global understanding of the virome of the transplanted lung is incomplete. To define the DNA virome within the respiratory tract following lung transplantation we carried out metagenomic analysis of allograft bronchoalveolar lavage (BAL), and compared with healthy and HIV+ subjects. Viral concentrates were purified from BAL and analyzed by shotgun DNA sequencing. All of the BAL samples contained reads mapping to anelloviruses, with high proportions in lung transplant samples. Anellovirus populations in transplant recipients were complex, with multiple concurrent variants. Quantitative polymerase chain reaction quantifica

SUBMITTER: Young JC 

PROVIDER: S-EPMC4276431 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature