Ontology highlight
ABSTRACT:
SUBMITTER: Vvedenskaya IO
PROVIDER: S-EPMC4277259 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20140601 6189
Transcription elongation is interrupted by sequences that inhibit nucleotide addition and cause RNA polymerase (RNAP) to pause. Here, by use of native elongating transcript sequencing (NET-seq) and a variant of NET-seq that enables analysis of mutant RNAP derivatives in merodiploid cells (mNET-seq), we analyze transcriptional pausing genome-wide in vivo in Escherichia coli. We identify a consensus pause-inducing sequence element, G₋₁₀Y₋₁G(+1) (where -1 corresponds to the position of the RNA 3' e ...[more]