Fourier transform infrared with attenuated total reflectance applied to the discrimination of freshwater planktonic coccoid green microalgae.

ABSTRACT: Despite the recent advances on fine taxonomic discrimination in microorganisms, namely using molecular biology tools, some groups remain particularly problematic. Fine taxonomy of green algae, a widely distributed group in freshwater ecosystems, remains a challenge, especially for coccoid forms. In this paper, we propose the use of the Fourier Transform Infrared (FTIR) spectroscopy as part of a polyphasic approach to identify and classify coccoid green microalgae (mainly order Sphaeropleales), using triplicated axenic cultures. The attenuated total reflectance (ATR) technique was tested to reproducibility of IR spectra of the biological material, a primary requirement to achieve good discrimination of microalgal strains. Spectral window selection was also tested, in conjunction with the fi

SUBMITTER: de Moraes GP 

PROVIDER: S-EPMC4277285 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature