High reproducibility of histological characterization by whole virtual slide quantification; an example using carotid plaque specimens.

ABSTRACT:

Objective

Tissue biobanks are an important source for discovery and validation studies aiming for new proteins that are causally related with disease development. There is an increasing demand for accurate and reproducible histological characterization, especially for subsequent analysis and interpretation of data in association studies. We assessed reproducibility of one semiquantative and two quantitative methods for histological tissue characterization. We introduce a new automated method for whole digital slide quantification. Carotid atherosclerotic plaques were used to test reproducibility.

Methods

50 atherosclerotic plaques that were obtained during carotid endarterectomy were analysed. For the semiquantitative analysis, 6 different plaque characteristics were scored

SUBMITTER: Vrijenhoek JE 

PROVIDER: S-EPMC4277457 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature