Murine abdominal aortic aneurysm model by orthotopic allograft transplantation of elastase-treated abdominal aorta.

ABSTRACT:

Objective

Murine models have proved instrumental in studying various aspects of abdominal aortic aneurysm (AAA), from identification of underlying pathophysiologic changes to the development of novel therapeutic strategies. In the current study, we describe a new model in which an elastase-treated donor aorta is transplanted to a recipient mouse and allowed to progress to aneurysm. We hypothesized that by transplanting an elastase-treated abdominal aorta of one genotype to a recipient mouse of a different genotype, one can differentiate pathophysiologic factors that are intrinsic to the aortic wall from those stemming from circulation and other organs.

Methods

Elastase-treated aorta was transplanted to the infrarenal abdominal aorta of recipient mice by end-to-side microsurg

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4277509 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature