A set of computationally designed orthogonal antiparallel homodimers that expands the synthetic coiled-coil toolkit.

ABSTRACT: Molecular engineering of protein assemblies, including the fabrication of nanostructures and synthetic signaling pathways, relies on the availability of modular parts that can be combined to give different structures and functions. Currently, a limited number of well-characterized protein interaction components are available. Coiled-coil interaction modules have been demonstrated to be useful for biomolecular design, and many parallel homodimers and heterodimers are available in the coiled-coil toolkit. In this work, we sought to design a set of orthogonal antiparallel homodimeric coiled coils using a computational approach. There are very few antiparallel homodimers described in the literature, and none have been measured for cross-reactivity. We tested the ability of the distance-depende

SUBMITTER: Negron C 

PROVIDER: S-EPMC4277747 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature